We have developed innovative solutions for optimizing the process of accurately quantifying proteins in complex biological matrices and other complex mixtures.
Isobaric Label services incorporate sample multiplexing to provide deep proteome coverage and highly precise quantitation of small or medium-sized sample sets. Samples are prepared and labeled separately, then all samples are pooled and analyzed by nano-flow LC-MS/MS1.
Multiplexed peptides elute as uniform chromatographic peaks and the peptide ions are analyzed using data-dependent selection and high field Orbitrap MS/MS detection. Peptide fragmentation yields specific reporter ions corresponding to each of the multiplexed channels.
We provide Isobaric Label Quantitative Proteomics service using Tandem Mass Tag (TMT) technology developed by Proteome Sciences plc and licensed by Thermo Fisher Scientific2.
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Isobaric Label Quantitative Proteomics services are performed using nano-flow liquid chromatography and high resolution Orbitrap mass spectrometry.
We accept protein samples in a variety of formats. For attaining maximum proteome coverage we recommend utilizing our sample fractionation services, performed using our off-line UHPLC-UV platform.
| Sample type | Amount and Concentration | Minimum sample volume | |
| Protein sample in liquid solution | Recommended | 1000 µg; 2 µg/µL | 250 µL |
| Minimum Required | 50 µg; 1 µg/µL | 50 µL | |
GO (Gene ontology) category analysis
COG (Cluster of Orthologous Groups of proteins) category analysis
Pathway analysis
DEPs (differentially expressed proteins) cluster analysis
DEPs GO enrichment analysis
DEPs pathway enrichment analysis
