Proteomics and metabolomics data are increasingly combined with genomics information in multi-omics studies to enhance basic research and drug development projects. BGI is a pioneer in the field of trans-omics and offers advanced proteomics and bioinformatics solutions to support our client’s research1,2.
Recent advances in mass spectrometry have provided powerful tools for the analysis of proteins from a variety of organisms and cell types. BGI applies state-of-the-art MS-based techniques for protein identification, protein characterization, relative and absolute quantitation, as well as for the study of post-translational modifications (PTMs) and protein-protein interactions.
Proteome Profiling
Proteome Profiling is a cost effective, high value solution for monitoring hundreds-to-thousands of proteins simultaneously. Our UHPLC-UV sample fractionation services are highly recommended for complex sample types to maximize protein sequence coverage and detection dynamic range3.
We perform sample digestion using sequencing-grade trypsin or alternative proteolytic method. Trypsin-digested peptide samples are analyzed using nano-flow LC-MS/MS. Spectral counting method provides approximate protein relative abundances4.
PTM Profiling
Our PTM Profiling service utilizes multiple protease digests run independently to provide high sequence coverage of detected proteins which is critical for confident PTM site identifications5.
Phospho Enrichment Profiling
Our Phospho Enrichment Profiling service can be customized to meet the needs of your drug discovery program.
Phosphorylated peptides are enriched using titanium dioxide. For deep phopho-proteome analysis off-line fractionation is highly recommended6. We provide a full data report detailing detected protein IDs, peptide-localized PTM assignments, and PSM counts.
References
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Proteome and PTM Profiling services are performed using nano-flow liquid chromatography and high resolution Orbitrap mass spectrometry.
We accept protein samples in a variety of formats. For maximum proteome coverage complex samples can be extensively fractionated off-line using our UHPLC-UV platform.
| Sample type | Amount and Concentration | Minimum sample volume | |
| Protein sample in liquid solution or in gel band/spot | Recommended | 100 µg for each enzyme; 2 µg/µL | 100 µL for each enzyme |
| Minimum Required | 10 µg for each enzyme; 1 µg/µL | 10 µL for each enzyme | |
Data analysis and validation performed with SEQUEST or Mascot
GO (Gene ontology) category analysis
COG (Cluster of Orthologous Groups of proteins) category analysis
Pathway analysis
DEPs (differentially expressed proteins) cluster analysis
DEPs GO enrichment analysis
DEPs pathway enrichment analysis
