We have developed innovative solutions for optimizing the process of accurately quantifying proteins in complex biological matrices and other complex mixtures.
Data-Independent Acquisition (DIA) is a label-free quantitative technique which provides highly consistent quantitation and broad proteome coverage1. We create a customized spectral library with your specific sample and then analyze individual samples using nano-flow LC-MS/MS with DIA scanning and quantitation2.
Label-Free DIA Quantitative Proteomics service is ideal for long-term projects or projects with large sample sets which require accurate and reproducible quantitation.
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Label-Free DIA Quantitative Proteomics services are performed using nano-flow liquid chromatography and high resolution Orbitrap mass spectrometry.
We accept protein samples in a variety of formats. For attaining maximum proteome coverage we recommend utilizing our sample fractionation services, performed using our off-line UHPLC-UV platform.
| Sample type | Amount and Concentration | Minimum sample volume | |
| Protein sample in liquid solution | Recommended | 400 µg with fractionation; 2 µg/µL | 200 µL |
| Minimum Required | 100 µg with fractionation; 1 µg/µL | 100 µL | |
GO (Gene ontology) category analysis
COG (Cluster of Orthologous Groups of proteins) category analysis
Pathway analysis
DEPs (differentially expressed proteins) cluster analysis
DEPs GO enrichment analysis
DEPs pathway enrichment analysis
