DNA Sample Requirement General Guidelines for:
For questions on submitting other sample types or for custom sequencing solutions, please contact our Project Management teams or your BGI Sales Representative to discuss further.
Contact Support: info@bgi.com
Sample type: genomic DNA/PCR product
Quality requirements: >1µg, >12.5ng/µl
Sample volume for QC: 1ul for Qubit, 100ng for Electrophoresis
Resuspension Buffer: Nuclease-free water, TE buffer (<0.1mM EDTA), EB
Shipment: Dry ice
Sample type: genomic DNA/PCR product
Quality requirements: >1.5µg, >20ng/µl
Sample volume for QC: 1ul for Qubit, 100ng for Electrophoresis
Resuspension Buffer: Nuclease-free water, TE buffer (<0.1mM EDTA), EB
Shipment: Dry ice
Sample type: genomic DNA/PCR product
Quality requirements: m>20µg, c>110ng/µl, no degradation or slight degradation
Sample volume for QC: 1ul for Qubit, 100ng for Electrophoresis
Resuspension Buffer: Nuclease-free water, TE buffer (<0.1mM EDTA), EB
Shipment: Dry ice
Sample type: genomic DNA
Quality requirements: m>30µg, c>110ng/µl, no degradation or slight degradation
Sample volume for QC: 1ul for Qubit, 100ng for Electrophoresis
Resuspension Buffer: Nuclease-free water, TE buffer (<0.1mM EDTA), EB
Shipment: Dry ice
Sample type: genomic DNA
Quality requirements: m>40µg, c>110ng/µl, no degradation or slight degradation
Sample volume for QC: 1ul for Qubit, 100ng for Electrophoresis
Resuspension Buffer: Nuclease-free water, TE buffer (<0.1mM EDTA), EB
Shipment: Dry ice
Sample type: genomic DNA
Quality requirements: m>50µg, c>110ng/µl, no degradation or slight degradation
Sample volume for QC: 1ul for Qubit, 100ng for Electrophoresis
Resuspension Buffer: Nuclease-free water, TE buffer (<0.1mM EDTA), EB
Shipment: Dry ice
Sample type: genomic DNA
Quality requirements: m>10µg, c>30ng/µl, no degradation or slight degradation
Sample volume for QC: 1ul for Qubit, 100ng for Electrophoresis
Resuspension Buffer: Nuclease-free water, TE buffer (<0.1mM EDTA), EB
Shipment: Dry ice.
Quality requirements: m>1µg, c>12.5ng/µl, no degradation or slight degradation
Sample volume for QC: 1ul for Qubit, 100ng for Electrophoresis
Resuspension Buffer: Nuclease-free water, TE buffer (<0.1mM EDTA), EB
Shipment: Dry ice.
Quality requirements: m>1µg, c>12.5ng/µl, main fragment size ≥250bp
Sample volume for QC: 1ul for Qubit, 100ng for Electrophoresis
Resuspension Buffer: Nuclease-free water, TE buffer (<0.1mM EDTA), EB
Shipment: Dry ice
Sample type: genomic DNA
Quality requirements: m>10µg, c>30ng/µl, no degradation or slight degradation
Sample volume for QC: 1ul for Qubit, 100ng for Electrophoresis
Resuspension Buffer: Nuclease-free water, TE buffer (<0.1mM EDTA), EB
Shipment: Dry ice
Quality requirements: m>1µg, c>12.5ng/µl, no degradation or slight degradation
Sample volume for QC: 1ul for Qubit, 100ng for Electrophoresis
Resuspension Buffer: Nuclease-free water, TE buffer (<0.1mM EDTA), EB
Shipment: Dry ice
Quality requirements: m>1µg, c>12.5ng/µl, main fragment size ≥250bp
Sample volume for QC: 1ul for Qubit, 100ng for Electrophoresis
Resuspension Buffer: Nuclease-free water, TE buffer (<0.1mM EDTA), EB
Shipment: Dry ice
Sample type: ChIP-ed DNA
Quality requirements: m>10ng, c>1ng/µl, Major Peak in 100bp-500bp (Agilent 2100)
Sample volume for QC: 1ul for Qubit, 2ul for Agilent 2100
Resuspension Buffer: Nuclease-free water, TE buffer (<0.1mM EDTA), EB
Shipment: Dry ice
Sample type: genomic DNA
Quality requirements: m>300ng, c>10ng/µl, no degradation or slight degradation, No RNA and Protein contamination
Sample volume for QC: 1ul for Qubit, 100ng for Electrophoresis
Resuspension Buffer: Nuclease-free water, TE buffer (<0.1mM EDTA), EB
Shipment: Dry ice
Sample type: genomic DNA
Quality requirements: m>1µg, c>20ng/µl, no degradation or slight degradation, No RNA and Protein contamination
Sample volume for QC: 1ul for Qubit, 100ng for Electrophoresis
Resuspension Buffer: Nuclease-free water, TE buffer (<0.1mM EDTA), EB
Shipment: Dry ice
Sample type: genomic DNA
Quality requirements: m>3µg, c>37.5ng/µl, no degradation or slight degradation, No RNA and Protein contamination
Sample volume for QC: 1ul for Qubit, 100ng for Electrophoresis
Resuspension Buffer: Nuclease-free water, TE buffer (<0.1mM EDTA), EB
Shipment: Dry ice
Sample type: genomic DNA
Quality requirements: m>1µg, c>15ng/µl, no degradation or slight degradation
Sample volume for QC: 1ul for Qubit, 100ng for Electrophoresis
Resuspension Buffer: Nuclease-free water, TE buffer (<0.1mM EDTA), EB
Shipment: Dry ice
Sample type: genomic DNA
Quality requirements: m>1µg, c>20ng/µl, no degradation or slight degradation
Sample volume for QC: 1ul for Qubit, 100ng for Electrophoresis
Resuspension Buffer: Nuclease-free water, TE buffer (<0.1mM EDTA), EB
Shipment: Dry ice
Sample type: ChIP-ed DNA
Quality requirements: m>10ng, c>1ng/µl, Major Peak in 100bp-500bp (Agilent 2100)
Sample volume for QC: 1ul for Qubit, 2ul for Agilent 2100
Resuspension Buffer: Nuclease-free water, TE buffer (<0.1mM EDTA), EB
Shipment: Dry ice
Sample type: genomic DNA
Quality requirements: m>1µg, c>12.5ng/µl, No degradation or slight degradation
Sample volume for QC: 1ul for Qubit, 100ng for Electrophoresis
Resuspension Buffer: Nuclease-free water, TE buffer (<0.1mM EDTA), EB
Shipment: Dry ice
Sample type: genomic DNA
Quality requirements: m>1µg, c>12.5ng/µl, No degradation or slight degradation
Sample volume for QC: 1ul for Qubit, 100ng for Electrophoresis
Re-suspension Buffer: Nuclease-free water, TE buffer (<0.1mM EDTA), EB
Shipment: Dry ice
Sample type: genomic DNA
Quality requirements: m>10.2ug, c>80ng/µl, no degradation or slight degradation
OD260/280:1.8-2.0, OD260/230:2.0-2.2, Qubit/OD>0.5
Sample volume for QC: 1ul for Qubit, 100ng for Electrophoresis
Resuspension Buffer: Nuclease-free water, TE buffer (<0.1mM EDTA), EB
Shipment: Dry ice
Sample type: genomic DNA
Quality requirements: m>10.2µg, c>40ng/µl, No degradation or slight degradation
OD260/280:1.8-2.0, OD260/230:2.0-2.2, Qubit/OD>0.5
Sample volume for QC: 1ul for Qubit, 100ng for Electrophoresis
Resuspension Buffer: Nuclease-free water, TE buffer (<0.1mM EDTA), EB
Shipment: Dry ice
Sample type: genomic DNA
Quality requirements: m>4.2µg, c>50ng/µl, No degradation or slight degradation
OD260/280:1.8-2.0, OD260/230:2.0-2.2, Qubit/OD>0.5
Sample volume for QC: 1ul for Qubit, 100ng for Electrophoresis
Resuspension Buffer: Nuclease-free water, TE buffer (<0.1mM EDTA), EB
Shipment: Dry ice
Sample type: genomic DNA
Quality requirements: m>1.7µg, c>50ng/µl, No degradation or slight degradation
OD260/280:1.8-2.0, OD260/230:2.0-2.2, Qubit/OD>0.5
Sample volume for QC: 1ul for Qubit, 100ng for Electrophoresis
Resuspension Buffer: Nuclease-free water, TE buffer (<0.1mM EDTA), EB
Shipment: Dry ice
Sample type: genomic DNA
Quality requirements: m>1.2µg, c>40ng/µl, no degradation or slight degradation
OD260/280:1.8-2.0, OD260/230:2.0-2.2, Qubit/OD>0.5
Sample volume for QC: 1ul for Qubit, 100ng for Electrophoresis
Resuspension Buffer: Nuclease-free water, TE buffer (<0.1mM EDTA), EB
Shipment: Dry ice
Sample type: genomic DNA
Quality requirements: m>0.52µg, c>20ng/µl, no degradation or slight degradation
OD260/280:1.8-2.0, OD260/230:2.0-2.2, Qubit/OD>0.5
Sample volume for QC: 1ul for Qubit, 100ng for Electrophoresis
Resuspension Buffer: Nuclease-free water, TE buffer (<0.1mM EDTA), EB
Shipment: Dry ice
RNA Sample Requirement General Guidelines for:
For questions on submitting other sample types or for custom sequencing solutions, please contact our Project Management teams or your BGI Sales Representative to discuss further.
Contact Support: info@bgi.com
Quality requirements: m>200ng, c>20ng/µl, RIN≥7.0 (Agilent2100 or equivalent)
Sample volume for QC: 2~3ul
Resuspension Buffer: Nuclease-free water, EB
Shipment: Dry ice
Quality requirements: m>200ng, c>70ng/µl, DV200≥30%, RIN≥2.0 (Agilent2100 or equivalent)
Sample volume for QC: 2~3ul
Resuspension Buffer: Nuclease-free water, EB
Shipment: Dry ice
Sample type: Total RNA
Quality requirements: m>1ug, c>20ng/µl, RIN≥7.0, 28s/18s≥ 1.0(23s/16s≥ 1.0) (Agilent2100 or equivalent)
Sample volume for QC: 2~3ul
Resuspension Buffer: Nuclease-free water, EB
Shipment: Dry ice
Sample type: Total RNA
Quality requirements: m>200ng, c>20ng/µl, RIN≥7.0, 28s/18s≥ 1.0 (Agilent2100 or equivalent)
Sample volume for QC: 2~3ul
Resuspension Buffer: Nuclease-free water, EB
Shipment: Dry ice
Sample type: Total RNA
Quality requirements: m>1ug, c>20ng/µl, RIN≥7.0, 28s/18s≥ 1.0 (Agilent2100 or equivalent)
Sample volume for QC: 2~3ul
Resuspension Buffer: Nuclease-free water, EB
Shipment: Dry ice
Sample type: Total RNA
Quality requirements: m>200ng, c>20ng/µl, RIN≥7.0, 28s/18s≥1.0 (Agilent2100 or equivalent)
Sample volume for QC: 2~3µl
Resuspension Buffer: Nuclease-free water, EB
Shipment: Dry ice
Sample type: Total RNA
Quality requirements: m>1µg, c>40ng/µl, RIN≥6.5(Plant/Fungi), RIN≥7(Other animal), 28S/18S≥1.0 (Agilent2100 or equivalent)
Sample volume for QC: 2~3µl
Resuspension Buffer: Nuclease-free water, EB
Shipment: Dry ice
Sample type: Total RNA
Quality requirements: m>200ng, c>20ng/µl, RIN≥7.0, 28s/18s≥ 1.0 (Agilent2100 or equivalent)
Sample volume for QC: 2~3ul
Resuspension Buffer: Nuclease-free water, EB
Shipment: Dry ice
Sample type: Total RNA
Quality requirements: m>1ug, c>40ng/µl, RIN≥7.0, 28s/18s≥ 1.0 (Agilent2100 or equivalent)
Sample volume for QC: 2~3ul
Resuspension Buffer: Nuclease-free water, EB
Shipment: Dry ice.
Quality requirements: m>1ug, c>50ng/µl, RIN≥8.0, 28s/18s≥1.5(23s/16s≥1.5) (Agilent2100 or equivalent)
Sample volume for QC: 2~3ul
Resuspension Buffer: Nuclease-free water, EB
Shipment: Dry ice
Quality requirements: m>100ng, c>5ng/µl, (Agilent2100 or equivalent)
Sample volume for QC: 2~3ul
Resuspension Buffer: Nuclease-free water, EB
Shipment: Dry ice
All extractions are performed to the best of BGI’s ability. The customer is charged for work performed regardless of outcome unless otherwise stated. Additional charges for procurement of kits may apply for select extractions.
Recommended Quantity: DNA≥10 (RNA≥5) FFPE slides
Thickness: 5µm-10 µm
Diameter: 15 mm×20mm
Shipment: submitted at room temperature/blue ice
Recommended Quantity: 106 cells
BGI does not provide cell culturing
Shipment: submitted on dry ice
Recommended Quantity (DNA Extraction): ≥300mg (for DNA Insert >2kb, Recommended Quantity ≥1g)
Recommended Quantity (RNA Extraction): ≥30mg
Shipment: submitted on dry ice
Recommended Volume (DNA Extraction): ≥1 mL (EDTA Tube)
Recommended Volume (RNA Extraction): 2.5 mL (whole blood, PAXgene Blood RNA Tube)
Shipment: Fresh submitted on Blue Ice, Frozen submitted on Dry Ice
Recommended Volume (DNA Extraction): ≥5 mL
Recommended Volume (RNA Extraction): ≥1 mL
Shipment: Dry Ice
Recommended Quantity: 50~100 mg (Solid)
Recommended Volume: 500uL (OMNIgene•GUT(OMR-200), DNA Genotek Collection tube)
Shipment: Solid submitted on dry ice, Collection tube submitted at room temperature/blue ice
Recommended Quantity: 50~150mg
Shipment: submitted on dry ice
Recommended Quantity: 2 tubes/sample, 1 swap/tube
Shipment: submitted on dry ice
BGI performs QC free of charge.
Agilent 2100 BioAnalyzer
RNA
√
Microplate Reader/Qubit
DNA
√
Gel Electrophoresis
DNA
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